Abstract

The influence of neuraminidase from Vibrio cholerae on evoked, extracellular potentials from rat brain striatal slices was tested. Enzyme treatment abolished synaptic transmission within 90 min. Biochemical analysis of the neuraminidase-treated slices has demonstrated a 10-fold increase in the amount of free sialic acid. The thin-layer chromatography of gangliosides extracted from enzyme-treated slices showed a decrease in the amount of polysialogangliosides (GD1a, GD1b, GT1b, GQ1b) by 1.5-fold. As a consequence, the amount of monosialoganglioside (GM1), which is resistant to neuraminidase from Vibrio cholerae, increased by 1.7-fold. As the antidromic potential remained unchanged after enzyme treatment, our results suggest involvement of polysialogangliosides in cholinergic transmission in the striatum.

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.