Abstract
1. 1. The production of 1,25 hydroxycholecalciferol (1,25-(OH) 2D 3) by dispersed kidney cells obtained from vitamin D or calcium deficient, and from vitamin D-fed or repleted birds, was evaluated. 2. 2. Once stimulated in vivo by vitamin D or calcium deficiency, the kidney cells continued the accelerated production of 1,25-(OH) 2D 3 for 3 hr, in vitro. 3. 3. Pure bovine parathyroid hormone and a homogenate, extract or TCA powder of chick parathyroid gland (PTG) increased significantly the production of 1,25-(OH) 2D 3 by kidney cells. 4. 4. The effect of avian PTG material on 24,25-(OH) 2D 3 (24,25-(OH) 2D 2) production was not significant. 5. 5. The effect of PTG homogenate was independent of calcium concentration in the incubation medium. A high calcium concentration (3.0 mM) somewhat reduced the formation in vitro of 1,25-(OH) 2D 3 but stimulated slightly the production of 24,25-(OH) 2D 3. 6. 6. The effect of parathyroid sources was significant in cells taken from vitamin D replete and not vitamin D deficient birds.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Comparative Biochemistry and Physiology -- Part B: Biochemistry and Molecular Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
