Abstract
Inside-out vesicles (10) were prepared from red cells obtained from cystic fibrosis (CF) patients and approximately aged-matched controls. Ca 2+-uptake activity was found to be significantly reduced in the preparations derived from CF patients. This reduction was apparent at all time points studied (10 sec-120 min) and at all free calcium concentrations used (10–150 μM). Reciprocal plots of the data revealed that the K diss for calcium of the calcium pump, was similar in control and CF samples but that the V Ca 2+ was significantly reduced (P<0.001) in the CF preparations. Calmodulin prepared from red cell hemolysates of controls was found to stimulate Ca 2+-transport activity to a similar extent in both CF and control samples.
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