Abstract
BackgroundKangfuxin (KFX) is the ethanol extract of Periplaneta americana L, which has been widely used in the Traditional Chinese Medicine for the repair and regeneration of injured organ and tissues with long history. This study is to investigate the influence of KFX in the various cellular activities and evaluate the anti-osteoporosis potential of KFX.MethodsThe influence of the KFX in the cellular activities, including: 1) migration, osteocalcin secretion of osteoblasts; 2) apoptosis of osteoclasts; 3) migration and tube formation of human umbilical vein endothelial cell (HUVEC); and 4) proliferation, cell cycle regulation and migration of bone marrow mesenchymal stem cells (BMSCs), were investigated systematically.ResultsKFX was shown to significantly 1) Promote of the migration of osteoblasts, HUVEC, and BMSCs; 2) Increase the secretion of osteocalcin and mineralization of osteoblasts; 3) Accelerate the apoptosis of osteoclasts; 4) Stimulate the proliferation and regulate the cell cycle of BMSCs.ConclusionTaken together, these results provide the evidence for the osteogenesis, anti-osteoporosis and angiogenesis effects of KFX, with the mechanism of activating the bone formation through stimulating the osteoblasts and HUVECs, as well as inhibiting the bone absorption by inhibiting the osteoclasts activities. The KFX was definitely shown a promising bone turnover agent with great potential for anti-osteoporosis treatment.
Highlights
Kangfuxin (KFX) is the ethanol extract of Periplaneta americana L, which has been widely used in the Traditional Chinese Medicine for the repair and regeneration of injured organ and tissues with long history
The migration of osteoblasts was monitored in relation to the closure of a denuded area scratched in a confluent monolayer
As time extended to 48 h, KFX (0.1, 1, 2 mg/mL) displayed a further enhancement in the osteoblasts migration (p < 0.05), consistent with that expressed by positive control (PC) substance (0.1 mg/mL, p < 0.05)
Summary
Kangfuxin (KFX) is the ethanol extract of Periplaneta americana L, which has been widely used in the Traditional Chinese Medicine for the repair and regeneration of injured organ and tissues with long history. Osteoporosis, the most frequent bone remodeling disease, is characterized by a low bone mass with susceptibility to fracture, which results in an increased risk of adverse health outcomes including decreased quality of life, There are some anti-osteoporotic drugs that could prevent and treat osteoporosis. Many of these drugs are associated with serious side effects. Huang et al BMC Complementary and Alternative Medicine (2017) 17:413 therapy were associated with headache and increased cancer risk such as breast cancer, endometrial cancer, and ovarian cancer [4, 5]. There is an urgent and increasing need for effective pharmacotherapy
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