Abstract
The association by aligned direct bonding of an evanescent wave optical sensor and small-volume flow cells for visible absorption detection is demonstrated. We show that for sample volumes ranging from 760 to 190 nl and a sensor interaction length of 1 mm, very small cross-sectional dimensions of fluid channels are no more a serious obstacle for optical detection. The lowest limit for the channel depth is the penetration depth of the evanescent wave, which is usually in the order of magnitude of a few micrometers.
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