Abstract
This study was aimed to evaluate the ability of local oyster mushroom(Pleurotus ostreatus; ID: MF065715.1; cultivated until maturation in growth medium containing cumin extract) to modulate the genotoxicity induced by cyclophosphamide (CP) in bone marrow cells of mice. In vivo genotoxicity was assessed by quantifying the incidence of micronucleated polychromatic erythrocytes in the bone marrow cells of mice that were administered different doses of P.ostreatus extract (150, 200,250, and 300 mg/kg/day( respectively, individually, and in combination with CP (40 mg/kg body weight) according to the following three experimental protocols (pre 2h, post 2h, and concomitant treatment for 14 days, respectively). Analysis and microscopic examination of micronuclei (MN) revealed no mutagenic effect of P.ostreatus extract alone at all the doses evaluated. By contrast, CP administration significantly increased (P<0.05) incidence of MN. Importantly, the co-administration of P.ostreatus extract with CP caused a significant and dose-dependent reduction in MN induced by CP in the murine bone marrow cells. These data suggest that P.ostreatus extract administration has a protective effect against genotoxic damage inflicted by CP. The dietary cumin may serve as a scavenger for free radicals generated by CP and may augment the antioxidant activity of P. ostreatus extract. These findings open up new avenues for the use of oyster mushrooms in many applications, including pharmacological preparations and food supplements.
Highlights
Cancer is one of the leading causes of death worldwide
Damage to healthy somatic tissues and immunosuppression are the common sideeffects that limit the use of CP (17).The creation of free radicals and other reactive oxygen species, in addition to lipid peroxidation, have been identified as the main mechanisms of CP-induced toxicity (19).CP is generally used as a genotoxic agent since CP and its metabolites can bind to and damage DNA, leading to chromosome breaks, micronuclei (MN) formation, and cell death(14)
Oxidative stress stems from an imbalance between the production of free radicals in our bodies, and the inability of our bodies to adequately counteract their damaging effects (6).One of the chemo-preventive strategies that can be readily incorporated into our daily lives to avert cancer is the utilization of natural compounds such as bioactive botanicals, food constituents,or functional foods (5).Mushrooms form an essential part of the normal human diet; they are a good source of a lot of minerals, digestible proteins, vitamins, and polysaccharides (12)
Summary
Cancer is one of the leading causes of death worldwide. Commonly-used anti-neoplastic treatments either prevent the proliferation of or kill tumor cells. Herbal antioxidants containing high concentrations of phenolic and flavonoid compounds, have been shown to have a strong chemo-protective influence against CP-induced DNA damage and oxidative stress in the bone marrow cells of mice (5,27). Their weight ranged from 25±2 g.They were assigned into six separate groups (as described in the “Experimental Design”section)under laboratory conditions These mice were housed in polypropylene cages and were provided food in the form of commercial mouse food pellets, and water ad libitum throughout the experiment Cyclophosphamide treatment Cyclophosphamide (Endoxan; 500 mg) was intra-peritoneal injection into mice at a dose equivalent to 40 mg/kg defined as a genotoxic dose for mouse bone marrow cells (4). G4- Pre-CP treatment :Mice (6 mice per mushroom extract dose; 24 mice total) were treated with four doses of P. ostreatus extract (150, 200, 250, and 300 mg/kg) delivered orally for 14 days.The animals were euthanized 2h after the treatment. Least significant difference –LSD test (ANOVA) was used to significant compare between means in this study
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
