Abstract
Sodium azide (NaN3) (2 mM) was used to induce mutation in black soybean variety CRWD for improvement of isoflavone content. The individual selections were made in M6, M7 and M8 generations, respectively. Both high levels of isoflavones and medium seed size traits were used as selection criteria. Across three growth seasons, the selected five NaN3-induced mutants accumulated more total isoflavones (average increases of 5% to 25%) than their wild type variety CRWD. Four mutants yielded more (average increases of 17% to 25%) than CRWD. Both mutants SA-M-03 (yellow cotyledon) and SA-M-05 (green cotyledon) are rich in isoflavones and may be useful in food and other applications.
Highlights
Isoflavones are a subclass of flavonoids derived from the phenylpropanoid pathway [1]
Because the isoflavone content of soybean seeds is affected by environments, breeding soybean cultivars with high levels of isoflavones in the classical manner is difficult [9]
A desirable soybean line with high isoflavone trait might be produced through mutation breeding
Summary
Isoflavones are a subclass of flavonoids derived from the phenylpropanoid pathway [1]. Isoflavones show significant antioxidant activities by inhibiting lipid peroxidation, scavenging free radicals and promoting anti-oxidative enzymes [5]. Due to these beneficial health effects, breeding varieties with high isoflavone content has been one of essential goals in soybean improvement programs [6]. A desirable soybean line with high isoflavone trait might be produced through mutation breeding. Mutation breeding of soybeans yielded in identification of many mutant lines with desirable traits, including resistance to pod shattering habit, low linolenic content and high oleic content [12,13]
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