Evaluation Yorkshire and Landrace breeds of pigs according to <i>PRKAG3</i>, <i>MC4R</i> and <i>MYOD1</i> marker genes

Abstract

Purpose: evaluation of pigs Yorkshire rocks and Landraz onPRKAG3, MC4RandMyOD1markers.Materials and methods. The paper presents the analysis of the results of genetic testing, and determines the frequency of alleles and genotypes of Yorkshire and Landrace breeds at the State Enterprise ZhodinoAgroPlemElita" of the Minsk region of the Republic of Belarus by the genes-markers of productive traits:PRKAG3, MC4RandMYOD1. Zootechnical and molecular genetic methods were used in the research (PCR/PDRF). Snapshot analysis was developed for the genotyping of pigs to detect polymorphic markers of meat-feeding productivity of pigs on the GenasPRKAG3, MyOD1, MC4R. At the first stage, there was an assessment of tribal young (173 heads) on its own productivity in terms of indicators: the age of achieving the living mass of 100 kg, the average daily increase (d) from birth to the live weight of 100 kg, the length of the body (cm), the lifetime meat quality of the thickness of the scrape (mm), height of the longest back muscles (mm), the content of meat in the body (%). At the second stage of the research, the controlling fastenings of 200 goals of pigs and evaluation of feedst and meat qualities in the following indicators were carried out: the age of achieving a living mass of 100 kg (days), the average daily increase (d), feed consumption per 1 kg of growth (to. Units). Also determined: the length of the carcass (cm), the slaughter output (%), the thickness of the spick over 6-7 breast vertebrae (mm), the area of "muscular eye" (cm2), the mass of the rear third of the half carcass (kg), the flow of meat in the carcus (%). Then, genetic studies were compared with productivity indicators.Results. As a result of the research, it was found that in the tested animals, the frequency of the preferred allele I-PRKAG3made 0.171-0.288, A-MC4R– 0.243-0.315, and C-MYOD1– 0.064-0.477, respectively. The developed comprehensive system for assessing the breeding value of Yorkshire and Landrace pigs based on the marker genesPRKAG3, MC4R, andMYOD1allowed us to identify the preferred alleles and determine correlation with productivity traits that provided increase in the average population values: for self-productivity - by 2-4%, for fattening traits - by 2.7-12.5%, for meat traits - by 2.8-34.4 %.Conclusion. The use of integrated system in pig breeding will speed up the selection work to increase the indicators of fattening and meat productivity of the created breeding herds of Yorkshire and Landrace pigs.