Evaluation urinary trehalase in patients with renal proximal tubular damage

Abstract

Objective To investigate the value of urinary trehalase（T） in patients with renal proximal tubular damage. Methods 134 patients with kidney disease (male:66 female:68 age:18-59; 31 cases with acute renal failure, 30 cases with chronic renal failure, 20 cases with drug-induced renal impairment, 21 cases with renal transplantation and 32 cases with nephritic syndrome) and 101 healthy controls (58 males and 43 females) were selected.Urinary T, N-acetyl-D-glucosaminidase (NAG), β2-MG, gamma-glutamyl transferase(GGT) were detected.Data were analyzed by SPSS 11.5, including non-parametric rank test, ROC analysis. Results The level of urinary trehalase in control group was normally distributed （7.1±4.1） μmol/h·g Cr (0-25 μmol/h·g Cr). There was no significant difference between men and women (t=0.63, P=0.53).Urinary T levels were significant higher in all kidney disease groups than in control group (Z=6.80,5.90, 5.23, 6.00, 8.04，P＜0.01).According to ROC curve, the area of urinary T under the ROC curve (AUC) in 134 patients was 0.9, significantly different with NAG, β2-MG, GGT area （P＜0.01），the AUCs of T were 0.94,0.85, 0.90,0.90,0.91 in acute and chronic renal failure group, drug-induced renal impairment group, renal transplantation group and nephritic syndrome group, respectively; Youden index were 0.85,0.65,0.77,0.66,0.72 respectively.Corresponding to the Youden index，sensitivity and specificity were 90.3% and 95.1%, 73.3% and 92.1%, 85.0% and 92.1%, 81.0% and 85.2%, 87.5% and 84.2% respectively. Conclusions The Urinary trehalase is better than other markers in the diagnosis of the proximal renal tubular damage.It was better to evaluate the proximal tubular function early in time.The diagnostic value of urinary trehalase played a key role in diagnosis, treatment and prognosis of kidney diseases.（Chin J Lab Med，2012,35：1135-1138） Key words: Kidney diseases; Kidney tubules; proximal; Trehalase; Urinalysis