Evaluation the efficiency of packing cucumber seeds with alginic acid loaded on Trichoderma koningii spores for time periods against pathogenic fungus Rhizoctonia solani

Abstract

This study was conducted at University of Agriculture and Veterinary Medicine in Bucharest laboratories, the concentration of 3.6*106 of T.koningii spores loaded on alginic acid was used to pack cucumber seeds at room temperature and for 6, 12 and 18 months against pathogenic fungus R.solani. Effectiveness of germination and seedling was evaluated every 6 months. The results of antagonism tests show that the bio fungus T.koningii have a high antagonism ability against the pathogen fungus R.solani, the antagonistic ratio 2 according to the scale of Bell. Also the results showed the effectiveness of germination of cucumber seeds packed with alginic acid loaded on T.koningii and contaminated with R.solani for 18 months of storage under room temperature, the rate of germination and seedling death 70.00% and 13.39%, respectively compared with non-loaded and contaminated seeds with R.solani which the rate reached to 30.00% and 55.55 % respectively. The best results of germination and seedling death were in cucumber seeds unloaded with alginic acid on T.koningii and contaminated with R.solani after 6 months which reached to 96.33% and 13.79 % respectively in comparison with cucumber seeds packed with alginic acid loaded on T.koningii and contaminated with R.solani which reached to 66.66% and 40.00% respectively.