Evaluation the effect of the use Immunoglobulin IgY immunity of infected chickens with the Eimeria tenella parasite

Abstract

Been obtained for the isolation of local Eimeria tenella by taking the Cecca of 850 samples from chickens slaughterhouses in the city of Ramadi, and after purification was diagnosed through the shape and size of the parasite and the infection , obtained the challenge strain by strengthening the isolation in Chicken type Ross 308 five times, injected challenge strain in laying hens Type Luhman under the skin and muscle of the chest and thigh after mixing with Freund's incomplete oil adjuvant solution, then get IgY from egg yolk, which began collected three days after the third dose, detection of IgY by using singular and double immunodiffusion test, use the IgY against Eimeria tenella in the treatment concentration of 1/4 with a dose of 5x 104 of the challenge strain and three replicates of treatment, showed the experiment results of the treated chicks with IgY, three days after giving the challenge dose has given the proportion of immunity amounted to 51.12%, while the results given the challenge dose with a solution of IgY given the proportion of immunity amounted to 75.55%, and finally give the challenge dose after giving the solution IgY in three days gave the proportion of immunity amounted 88.88% without any damage in chicks treated in the experiment compared with the control treatment of positive and which gave the proportion of immunity amounted 95.55% compared to control negative, which gave the proportion of immunity amounted 17.78%, as demonstrated by the study of statistical significant differences at the level of probability of 0.05 for all transactions except for the first treatment with the treatment of five .