Evaluation the Biological Activity of Nano- Gentamicin Prepared and Characterization of Nanoparticles

Rana Kamal,Ahmed J Essa,Awatif I Mohammed,Essraa A Mohamad,Quraish A Kahdhum,Maan A Abd Elhamid

doi:10.53523/ijoirvol8i2id79

Abstract

Gentamycin is a broad-spectrum antibacterial against Gram-positive and Gram-negative bacteria. Gentamycin Sulfate is a white powder that is freely soluble in water and insoluble in alcohol, acetone, chloroform and ether. The study aims to determine the characterization and the biological activity of gentamicin in the Nano-form prepared by Sol-gel application using an ultrasound device by aqueous solution at temperature of 80°. Nano gentamycin examined by Scanning electron microscopy (SEM), transmission (TEM) and the antibacterial effect test of both Nano and stander gentamycin were analyzed. The results showing that the average size of gentamycin nanoparticles is 68.51 nanometers with homogeneous distribution. The antibacterial effect of Nano gentamycin take a wide inhibition range of Gram-positive and Gram-negative bacteria compared to the standard substance, the inhibition diameters are for E. coli (29 mm) and for St. epidermidis (27 mm). In the other side the inhibitory ability for standard gentamicin for St. epidrmidis and E. coli were 18 mm and 20 mm, respectively.

Highlights

Gentamycin Sulphate: the antibiotic Gentamycin, which is an antibiotic has activity against gram-negative and gram-positive and gram-positive bacteria
Characterization Measurement of Nano- Gentamicin Atomic Force Microscopy To observe the surface roughness and topography of the sedimentary thin films, they are examined with a Scanning Probe Microscope (SPM), which was examined at Baghdad University / College of Science [10]
Characterization of Nano – Gentamicin Atomic Force Microscopy (AFM) The surface characteristic of gentamicin nanoparticles was determined by atomic force microscopy (AFM)

Summary

Introduction

Gentamycin Sulphate: the antibiotic Gentamycin, which is an antibiotic has activity against gram-negative and gram-positive and gram-positive bacteria. The study aims to determine the characterization and the biological activity of gentamicin in the Nano-form prepared by Sol-gel application using an ultrasound device by aqueous solution at temperature of 80°. The results showing that the average size of gentamycin nanoparticles is 68.51 nanometers with homogeneous distribution.

Results

Conclusion