Abstract
This study was aimed to evaluate the anti-oxidant activity of mmthanol extracts from roasted and unroasted sesame seeds cake (RSC, URSC) and ethanol extracts from sesameccoat (SC) usingDDPPH and reducing power activity (RPA) assay. The quantitative and qualitative analysis of obtained extracts were done using RP-HPLC technique, the results revealed that the extracts were contained anti-oxidents(lignans ) sesamin ,ssesamolin andssesamol in different amounts Also the RPA of URSC extract at (10, 20, & 50 mg/ml) was similar to that ofbBHT , while that for RSC was similar to BHT at 30 mg/ml. Additionally, The RPA SC extract was comparable to that of BHT at ( 20 , 30 , and 50 mg/ml). It has been noticed that the radical scavengingaability (RSA) of BHT was greater than of the experimental samples that evaluated by DPPH assay. Mean time the RSA of the studied extracts was increased with increase of extracts concentrations from 10-50 mg/g. Based on the results of this study, sesame coat, sesame cake (from roasted and unroasted sesame seeds) extracts could be used asppotential natural anti-oxidant topprotectooil-rich food to avoid theppossible risks resulted from using the syntheticaantioxidants to prevent food.
Highlights
The solutions were kept at room temperature for330 min and absorbance was measured at 517 nm against a blank using spectrophotometer.The radical scavenging activities (%) were calculated according to the following equation: DPPH radical scavenging activity (%) =
The superiority of URSC in sesame coat and sesame cake from roasted and reducing power could be attributed to the unroasted sesame seeds extracts and compared presence of sesamol in higher amount as to that of butylated hydroxytoluene (BHT)
Sample under study showed a concentrations Chang et al,(2) reported that the reducing dependent increases, as the concentration power of the sesame coats increased the reducing ability increased. It has extractiiiiiiiincreased withiiincreasing amount been noticed that the reducing power activity of extract, these results were agreed with Xie of sesame coat and RSC at all concentrations et al[20] who reported that the extract
Summary
Sesame cake was obtained from roasted and unroasted sesame seeds by extracting the oil as described by Hadeel and Khalid [4]
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