Evaluation the anti-proliferative effect of Pistacia atlantica gum extract as a natural compound on the human cervical cancer cell line (TC1 cells)

Abstract

Background: This survey was aimed at investigating the anti-proliferative impact of Pistacia atlantica gum extract as a natural compound on cervical cancer associated with papillomavirus. Methods: Aqueous extract of dried and milled Pistacia atlantica gum extract was assembled and evaluated by GC-MS. The MTT test was utilized for evaluating the antiprolifrative impacts of Pistacia atlantica gum extract (100, 200, and 400mg/ml). Furthermore, the estimation of perniciousness was possible via the survival ability of cells, and ROS production, apoptosis, and levels of caspase-8, and -9 proteins were specified. Results:A considerable (p<0.01) reduction in proliferation indicator (70.138 ± 8.464), a considerable (p <0.01) increase in apoptosis (71.66% ± 4.041) of TC1 cells, a noticeable (p <0.03) increase in ROS formation (15.69 ± 0.799) and a noticeable (p <0.01) increase in LDH release (17.83 ± 0.772) were evident in the adjacency of TC1 cells by a Pistacia atlantica gum extract (400 mg/ml) witha dose-dependent behavior in compared to control group. Moreover, a considerable (p < 0.05) increase was recorded in the activities of caspase-8 (0.097 ± 0.007) and caspase-9 (0.065 ± 0.004). Conclusion: The growth of TC1 cells could be suppressed, and cell apoptosis via could be evoked by the aqueous extract of Pistacia atlantica gum through extrinsic death-receptor-dependent apoptosis as well as intrinsic mitochondrial-dependent apoptosis. Therefore, the aqueous extract of Pistacia atlantica gum may be used as a natural compound for the treatment of disorders of papillomavirus.