Evaluation the Ability to Isolate and Culture Stem Cells from Frozen Human Umbilical Cord Tissue

Abstract

Umbilical cord-derived mesenchymal stem cells have been determined to be effective in cell therapy and regenerative medicine. Cell cryopreservation is widely used for long term preservation. However, cell cryopreservation is costly and labor consume. Therefore, cryopreservation of human umbilical cord (HUC) tissue has become an alternative solution to reduce the risks of disease and open up opportunities for later treatment for families in recent years. In this study, we evaluated the ability to isolate and the characteristic of mesenchymal stem cells (MSCs) derived from fresh and frozen human umbilical cord tissue. The results showed that the HUCMSCs were isolated successfully from both fresh and frozen HUC tissues. The expression of MSCs cell surface marker CD73, CD90, CD105 in group 1 were higher than those in group 2, CD73 (99,42 vs 95.2%); CD90 (99.74 vs 96.6%); CD105 (99.64 vs 96.15%), respectively. However, they are similar in morphology and osteogenic and adipose differentiated ability. The results of the study would be useful for stem cell research, cell therapy and regenerative medicine.