Abstract
Entamoeba histolytica is the causative agent of amoebiasis. This disease results in 40,000 to 100,000 deaths annually. The pathogenic molecules involved in the invasion of trophozoites had been constantly being clarified. This study explored the role of elongation factor 1 alpha (EF1a) in E. histolytica pathogenicity. Biolayer interferometry binding and pull-down assays suggest that EF1a and intermediate subunit of lectin (Igl) binding are specific. Submembranous distribution of EF1a closely aligns with the localization of Igl, which appear in abundance on membranes of trophozoites. Messenger RNA (mRNA) expression of EF1a is positively correlated with trends in Igl levels after co-incubation with Chinese hamster ovary (CHO) cells in vitro, suggesting a regulatory linkage between these proteins. Erythrophagocytosis assays also imply a role for EF1a in phagocytosis. Finally, EF1a and actin are collocated in trophozoites. These results indicated elongation factor 1a is associated with E. histolytica phagocytosis, and the relationships between EF1a, Igl, and actin are worth further study to better understand the pathogenic process.
Highlights
Entamoeba histolytica (E. histolytica) causes amoebiasis, a serious public health problem in both developing and developed countries
Endeavor, which showmight that Elongation factor 1 alpha (EF1a) to be efficacious consider that it is both essential for histolytica survival and known to be involved could bind to pathogenic lectin Igl and co-localization with actin
One report provides the use of E. histolytica EF1a in analyzing parasite taxonomic relationships [19], and a second provides the deduced amino acid sequence and major functional domains of the factor [18]
Summary
Entamoeba histolytica (E. histolytica) causes amoebiasis, a serious public health problem in both developing and developed countries. Acid deletions in EF1a have been found in some signal There transduction, tumorigenesis, and the cytoskeletal pathogenic protozoans of major global health importance, including [10,11], There are an interesting fact that the 12 amino acid deletions in Leishmania. We investigated molecular cloning and expression of EF1a and Igl in the these pathogens could have far-reaching benefits. Igl in the Several approaches arecellular currently in progress, attempting to develop pathogenic molecules related to trophozoites, analyzed localization of these proteins, and explored erythrocyte phagocytosis. Endeavor, which showmight that EF1a to be efficacious consider that it is both essential for histolytica survival and known to be involved could bind to pathogenic lectin Igl and co-localization with actin. Consider providing a basis strategy for novel target survival development for treatment prove to be efficacious that it is both essential for E.drug histolytica and known to be amoebiasis. Providing a basis strategy for novel drug target development for treatment amoebiasis
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