Evaluation of Wound Healing Activity of Salvianolic Acid B on In Vitro Experimental Model.

Urszula Szwedowicz,Krzysztof Gołąb,Anna Szewczyk,Anna Choromańska

doi:10.3390/ijms22147728

Urszula Szwedowicz, Krzysztof Gołąb + Show 2 more

Open Access

https://doi.org/10.3390/ijms22147728

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Abstract

Despite a wide range of bactericides and antiseptics, the treatment of chronic or complicated wounds is still a major challenge for modern medicine. Topical medications are the most sought-after new agents for use as treatment. The therapeutic concentration of their active substances is easy to achieve with the lowest possible burden on the patient’s body. This study assesses the effect of salvianolic acid B (Sal B) on the proliferation, migration, and production of collagen type III by fibroblasts, which are the most important processes in wound healing. The study was conducted on human gingival fibroblasts obtained from primary cell culture. The results showed that Sal B at a dose of 75 µg/mL increases the cell viability with significant stimulation of the cell migration as demonstrated in the wound healing assay, as well as an increase in the expression of collagen type III, which has great importance in the initial stages of wound scarring. The results obtained in the conducted studies and previous scientific reports on the antibacterial properties and low toxicity of Sal B indicate its high potential in wound healing.

Highlights

Despite many advances over the century in antibacterial pharmacotherapy and wound healing, still, several types of wounds are difficult to treat and nurture
In many chronic medical conditions such as diabetes, vascular diseases, or immunodeficiencies seemingly harmless injuries can quickly turn into complicated wounds [1,2]
The obtained results indicate that salvianolic acid B has a stimulating effect on human fibroblast cells

Summary

Introduction

Despite many advances over the century in antibacterial pharmacotherapy and wound healing, still, several types of wounds are difficult to treat and nurture. In many chronic medical conditions such as diabetes, vascular diseases, or immunodeficiencies seemingly harmless injuries can quickly turn into complicated wounds [1,2]. The pathophysiology of these diseases disrupts the already complex wound healing process which slows down at the stage of inflammation and the formation of granulation tissue [3]. The presence of exudate and its components has a degrading effect on proteins, extracellular matrix, and growth factors make wound closure impossible [5]. The main goal of wound care is to promote wound healing in the shortest possible time with minimal side effects

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