Abstract
ABSTRACT The present study deals with the fluorescent constituents of Almiros (or Almyros) River in Heraklion, Greece. Water samples have been pre-concentrated by the solid phase extraction (SPE) method and subjected to reversed phase high performance liquid chromatography (RP-HPLC) coupled with a fluorescence detector. Seasonal variations of organic matter were mainly observed while spatial variations were observed in a less extent. The samples from upstream points correspond to more fluorescence peaks and of higher intensity compared to the rest of the samples. Generally, the fluorescence intensity decreases towards downstream. Fluorescent intensities of peaks present an inversely proportional relation to the salinity. In concluding, fluorescence compounds may comprise a well indicator of the salinity fluctuations in well-developed karstification systems below sea level and open to the sea that present high mean flow rates with strong seasonal variability.
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