Evaluation of water management on arsenic methylation and volatilization in arsenic-contaminated soils strengthened by bioaugmentation and biostimulation

Abstract

Arsenic (As) fate in paddy fields has been one of the most significant current issues due to the strong As accumulation potential of rice plants under flooded conditions. However, no attempt was done to explore As methylation and volatilization under non-flooded conditions. Herein, we investigated the effects of water management on As methylation and volatilization in three arsenic-contaminated soils enhanced by biostimulation with straw-derived organic matter and bioaugmentation with genetic engineered Pseudomonas putida KT2440 (GE P. putida). Under flooded conditions, the application of biochar (BC), rice straw (RS) and their combination (BC+RS) increased total As in porewater. However, these effects were greatly attenuated under non-flooded conditions. Compared with RS amendment alone, the combination of GE P. putida and RS further promoted the As methylation and volatilization, and the promotion percentage under non-flooded conditions were significantly higher than that under flooded conditions. The combined GE P. putida and RS showed the highest efficiency in As methylation (88 µg/L) and volatilization (415.4 µg/(kg·year)) in the non-flooded soil with moderate As contamination. Finally, stepwise multiple linear regression analysis presented that methylated As, DOC and pH in porewater were the most important factors contributing to As volatilization. Overall, our findings suggest that combination of bioaugmentation with GE P. putida and biostimulation with RS/BC+RS is a potential strategy for bioremediation of arsenic-contaminated soils by enhancing As methylation and volatilization under non-flooded conditions.