Evaluation of Vitamin D3-Conjugated Selenium Nanoparticles (Se-NPs) on White Blood Cells, Liver, and Kidney Functions in Induced Immunosuppressed Rats

Abstract

The research was aimed of the to associate selenium nanoparticles (Se-NPs) previously synthesized using Aspergillus niger with vitamin D3 and to determine the effect of oral administration of each of them on some blood picture parameters and liver and kidney function parameters in laboratory rats in which immunosuppression was induced. The results showed that the color of the solution containing vitamin D3 with selenium nanoparticles (Se-NPs) changed from orange to pale as an initial indication of the coupling of Se-NPs with D3. Absorption UV-Visible Light Spectroscopy and their sizes are at 52.90 nm when measured using the X-ray diffraction technique, and the scanning electron microscope (SEM) showed that they are in the form of irregular spherical granules, whose sizes ranged from 5 to 135 nm. The state of immunosuppression of male laboratory rats using Sandimmune caused a negative effect in the significant reduction (p<0.05) of the numbers of white blood cells that were at 2230 (Cell × 106), as well as in the impairment of liver and kidney functions, and their levels increased significantly. Se-NPs (T3) or vitamin D3 (T4) alone or their conjugate solution (T5) modified the numbers of white blood cells, which became 6080, 4871, and 7164 (Cell × 106), respectively. It also caused a significant modification of the parameters of kidney and liver functions and returned them to their normal levels in the control group.