Evaluation of Virucidal Efficacy of Cold Plasma on Bacteriophage Inside a Three-Layered Sterilization Chamber

Abstract

Traditional disinfection methods against pathogens have numerous shortcomings, and inventive methods like cold plasma are required for virus inactivation. Here, an atmospheric pressure 13.56 MHz radio-frequency hollow cathode (RF–HC) cold plasma device has been used to build a cold plasma sterilization device, and its virucidal activity is assessed against P2 bacteriophage, a model surrogate for pathogenic viruses. The heart of this device contains a three-layered sterilization chamber which is a rectangular parallelopiped of 42 × 32 × 30 cm3. Optimization experiments were performed to make each corner of this chamber completely virus free after cold plasma treatment. This two-pronged study was conducted to establish the requirement of minimum vol. % H2O2 in minimum time for the complete elimination of phages inside this sterilization chamber even when plasma is not in the direct line of sight. In initial experiments, the effect of the direct plasma line of sight was seen as the top and bottom layers showing less phage killing as compared to the middle of the sterilization chamber. Complete sterilization of bacteriophage, in all the three layers inside the sterilization chamber, was achieved by plasma treatment with 6% H2O2 for 10 min in 80 watts of plasma operating power. It was also seen that 6% H2O2 mist alone is not sufficient to provide a high degree of sterilization, and normal water mist combined with cold plasma can provide a higher level of sterilization at each corner of the chamber.