Evaluation of ViroTrack Sero Zika IgG/IgM, a New Rapid and Quantitative Zika Serological Diagnostic Assay.

Tony Liao,Marco Donolato,Magelda Montoya Cruz,Eva Harris,Angel Balmaseda,Xiaole Wang,Robert Y.L Wang

doi:10.3390/diagnostics10060372

Tony Liao, Marco Donolato + Show 5 more

Open Access

https://doi.org/10.3390/diagnostics10060372

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Abstract

Dengue virus (DENV) and Zika virus (ZIKV) belong to the flavivirus genus and are antigenically closely related. They also share the same mosquito vector and can cause similar symptoms upon infection. However, DENV and ZIKV infections lead to different clinical sequelae and treatments; therefore, clinicians need rapid and accurate diagnostics capable of distinguishing between the two diseases. Methods: We employed the immuno-magnetic assay technology on a microfluidic cartridge (ViroTrack Sero Zika IgG/IgM) for diagnosis of ZIKV infection based on the aggregation of magnetic nanoparticles. We carried out three serological studies including samples from the Dominican Republic, USA, and Nicaragua, aimed at detecting ZIKV-specific IgG and IgM using the ViroTrack Sero Zika IgG/IgM test. Results: The seroconversion results were comparable with ZIKV IgG and IgM reactivity measured by the commercial ZIKV ELISA kit. The sensitivity and specificity for both ZIKV IgG and IgM tested by the ViroTrack Sero Zika IgG/IgM was approximately 98% and 93%, respectively. Conclusion: Serological detection of ZIKV infection by the new ViroTrack Sero Zika IgG/IgM test shows promising performance and limited cross-reactivity with DENV.

Highlights

Zika virus (ZIKV), an arthropod-borne virus belonging to the flavivirus genus in the Flaviviridae family, became a global epidemic after its introduction into Brazil in 2014–2015 [1]
Evaluation of ViroTrack Sero Zika IgG/IgM for detection of ZIKV-specific IgG and IgM was performed in three sample sets
In serological study 2, the sensitivity and specificity of the ViroTrack Sero Zika IgG/IgM cartridge was compared to standard techniques in a group of

Summary

Introduction

Zika virus (ZIKV), an arthropod-borne virus (arbovirus) belonging to the flavivirus genus in the Flaviviridae family, became a global epidemic after its introduction into Brazil in 2014–2015 [1]. It spread rapidly throughout the Americas and caused severe clinical sequelae such as Guillain-Barré Syndrome (GBS) in adults, and microcephaly and other congenital defects in babies born to mothers infected during pregnancy in Oceania. ZIKV is mainly transmitted by Aedes genus mosquitoes [2,3]. ZIKV was first identified in Uganda in 1947 in monkeys [11]

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