Evaluation of vessel density in healthy subjects with family history of glaucoma.

Abstract

To investigate whether any microvascular changes are present in optic disc, peripapillary or maculary regions in healthy subjects with a family history of glaucoma. A total of 82 healthy subjects including 42 first-degree relatives of primary open-angle glaucoma (POAG) and 40 controls were enroled in this cross-sectional study. Global and sectoral vessel density (VD) measurements excluding large vessels, retina nerve fibre layer (RNFL) and macula ganglion cell (mGCC) thicknesses were obtained from a combined optical coherence tomography-angiography (OCT-A) and spectral-domain OCT system. Effect size (ES) was used for evaluating the magnitude of the statistically significant difference. Area under receiver operating characteristic curves (AUCs) were used to examine the ability of parameters to differentiate first-degree relatives to controls. There was no significant difference in RNFL and mGCC thicknesses between groups. Although lower VD measurements in optic disc, peripapillary region, and macula were observed in the first-degree relatives, statistically significant mean difference (3.13 ± 0.87, p = 0.001) and large ES (0.80) were in only lower nasal sector of peripapillary region. Inter-eye asymmetry of supero-nasal VD was also statistically higher (3.74 ± 2.55 vs 1.89 ± 1.64) with a large ES in the first-degree relatives (p < 0.001, ES = 0.86). AUC for differentiating first-degree relatives from controls was highest for inter-eye asymmetry of supero-nasal sector VD (0.74, p < 0.001), followed by lower nasal sector VD (0.72, p < 0.001). Compared to controls, first-degree relatives of patients with POAG were found to have significantly greater inter-eye asymmetry in supero-nasal peripapillary VD and less VD in the lower nasal peripapillary region.