Abstract

Growth of five strains of mycelial and yeast phases of Sporothrix schenckii and Blastomyces dermatitidis , five strains of mycelial and three strains of yeast phases of Histoplasma capsulatum , five strains of mycelial phase of Coccidioides immitis , and five strains each of Nocardia asteroides and Cryptococcus neoformans was evaluated on brain heart infusion (BHI), heart infusion (HIA), and trypticase soy (TSA) agars containing human and sheep blood at concentrations of 5 and 10%. BHI and HIA were equally efficient in supporting growth of the organisms tested. Varying the amount of blood did not have an appreciable effect on growth. For the conversion or maintenance of the yeast phases of the dimorphic fungi, human blood was more efficient than sheep blood; however, in all other aspects studied they appeared to be comparable. TSA with sheep or human blood was clearly inferior to the other media studied.

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