Abstract
In recent years, photosynthetic microalgae regained attention for biodiesel production. For efficient utilization of microalgae, a number of criteria including a strain with high biomass and lipid productivities and employment of effective and reliable methods for oil extraction from the obtained biomass should be met. Recently, we have isolated and identified three thermo-resistant green microalgae strains, namely; Scenedesmus sp. ME02, Hindakia tetrachotoma ME03 and Mic-ractinium sp. ME05. In this study, we compared percent lipid content of thermos-tolerant mic-roalgal strains using the following solvent extraction methods: Soxhlet, Bligh and Dyer and Folch methods with or without assisted cell disruption techniques including lyophilization, homogenization, ultrasonication, bead and microwave-assisted. The highest increase in lipid yield was obtained with a combination of lyophilization and ultrasonication techniques together with Soxhlet method: 27% of total dry weight for Micractinium sp. ME05. We conclude that lyophilization and ultrasonication are effective assistance methods for lipid extraction from thermo-resistant microalgae.
Highlights
The global demand for fuels is at all time high due to the ever-increasing world population and energy consumption associated with it [1]
We identified these strains as potential candidates for large scale biodiesel production
We evaluated efficiencies of three solvent extraction methods, namely Soxhlet extraction, Bligh and Dyer and Folch methods with or without assisted cell disruption techniques including lyophilization, homogenization, ultrasonication, bead-assisted and microwave-assisted for lipid extraction from these microalgae
Summary
The global demand for fuels is at all time high due to the ever-increasing world population and energy consumption associated with it [1]. Majority of world fuel demand is supplied by fossil-based fuels [2] This type of fuel is problematic in many respects, because of its detrimental effects on the environment and needs to be replaced by renewable energy sources in the near future [3] [4]. Photosynthetic microalgae are advantageous as an alternative source of biodiesel with little or no competition for arable land, adaptability to different growth environments, high biomass accumulation and lipid yield [5]. Oil content of some microalgae can be as high as 80% of dry weight [6] Despite these advantages, microalgae are yet not commercially competitive with petroleum based fuel [1] [7]. Prior to large scale cultivation, each parameter needs to be optimized per microalgal strain
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
