Abstract
Several rapid methods have been developed for the enumeration of yeast and mould in foods. Among these rapid methods, the NEO-GRID hydrophobic grid membrane filter (HGMF) requires a considerably short incubation period and the TEMPO YM offers automation. TEMPO YM and NEO-GRID HGMF were compared with the current Food and Drug Administration (FDA) method, the reference method, for the enumeration of yeast and mould in foods. This study of method comparison was conducted using 261 naturally contaminated food samples including dried fruits, marmalade, flour, pastry, sausage, nuts, sauce and spices. The results of the statistical analysis of different food categories demonstrated that differences between the rapid methods and the FDA method were not significant (P≯0.05). Both TEMPO YM and NEO-GRID HGMF are rapid and efficient alternative methods for the enumeration of yeast and mould in foods.
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