Evaluation of two point-of-care meters and a portable chemistry analyzer for measurement of blood glucose concentrations in juvenile white-tailed deer (Odocoileus virginianus)

Abstract

To evaluate agreement of blood glucose concentrations measured in juvenile white-tailed deer (Odocoileus virginianus) by use of 2 point-of-care (POC) blood glucose meters and 1 portable chemistry analyzer with values obtained in serum by use of a standard laboratory chemistry analyzer, and to evaluate agreement between results obtained with the 2 POC meters. Prospective evaluation study. 14 venous blood samples from 14 healthy white-tailed deer fawns. Blood glucose concentration was measured with each of 2 POC meters. The remainder of the sample was divided into 2 tubes (1 that contained lithium heparin and 1 with no anticoagulant). Glucose concentration in anticoagulated whole blood was measured with the portable analyzer. Serum was collected from the remaining sample for measurement of glucose concentrations with the laboratory analyzer. Bland-Altman plots were used to assess agreement. Agreement between POC blood glucose meters and the laboratory analyzer was poor; mean values for bias were 2.9 mg/dL (95% limits of agreement [LOA], -70.2 to 76.0 mg/dL) and -30.8 mg/dL (95% LOA, -111.6 to 49.9 mg/dL), respectively. Agreement between the 2 POC meters was also poor (bias, 31.0 mg/dL; 95% LOA, -47.2 to 109.2 mg/dL). Agreement between the portable analyzer and the laboratory analyzer was good (bias, -1.6 mg/dL; 95% LOA, -15.3 to 12.1 mg/dL). Results suggested that the POC blood glucose meters used in this study are not appropriate for measurement of blood glucose concentrations in juvenile white-tailed deer.