Evaluation of Two Parts of Lithocarpus polystachyus Rehd. from Different Chinese Areas by Multicomponent Content Determination and Pattern Recognition.

Chih-Ching Huang,Min Wei,Qi Deng,Cuiying Shi,Ye Zhang,Xuexian Chen,Yangling Tuo,Xu Zhang

doi:10.1155/2020/8837526

Abstract

The purpose of this work is to establish a new method using high-performance liquid chromatography-diode array detection (HPLC-DAD) with chemometrics analysis to determine the content of catechin, isoquercetin, astragalin, phloridzin, trilobatin, and phloretin for one flavanol and five flavonoids, filter out the key compounds, and evaluate the quality of 26 batches of tender leaves and flower spikes of Lithocarpus polystachyus Rehd. (LP) from ten areas in China. The result showed that the HPLC-DAD method had excellent performance for accurate quantification analysis. S3 (tender leaf from Lushan, Sichuan) had the highest contents for six measured chemicals with trilobatin content of up to 27.82% in dry weight. S22 (flower spike from Liangping, Chongqing) had the highest content of phloridzin (up to 7.28%). All samples were divided into three types based on spatial distribution using principal component analysis. The result showed that the tender leaves and flower spikes from the same areas had many similar properties, and there were significant differences between the samples from different regions. Furthermore, phloridzin and trilobatin were identified as chemical markers for quality evaluation of two parts with different tender leaves and flower spikes of LP from geographical areas by orthogonal partial least squares discrimination analysis. These results will be helpful to establish an effective and comprehensive evaluation system of the development and utilization of LP resources.

Highlights

Lithocarpuspolystachyus Rehd. (LP) belongs to the Lithocarpus arbor of the Fagaceae family and is widely distributed in some Asian countries, including China, ailand, and India [1]
There is no study on the quality divergence or quantification of components of the tender leaves and flower spikes of LP, which is unfavorable to evaluate the quality of LP and standardize market products
Considering the low contents of catechin, isoquercetin, and astragalin in LP, methanol is selected as the extraction solvent. e extraction amount of the components increases with the increase of the solvent volume and ultrasonic time but tends to slow down after increasing to a certain extent. erefore, 25 mL methanol and 25 min for ultrasonication are selected as the extraction conditions

Summary

Introduction

Lithocarpuspolystachyus Rehd. (LP) belongs to the Lithocarpus arbor of the Fagaceae family and is widely distributed in some Asian countries, including China, ailand, and India [1]. The National Health Commission of the People’s Republic of China has accepted LP as a new food raw material since 2017. LP has been widely used in health care foods, medicine, and other fields. As a new food raw material, LP has huge economic and medical values, which have been artificially cultivated in Hunan, Jiangxi, Chongqing, Sichuan, and other provinces in China [5]. The products of the tender leaves and flower spikes of LP have appeared in the Chinese market. There is no study on the quality divergence or quantification of components of the tender leaves and flower spikes of LP, which is unfavorable to evaluate the quality of LP and standardize market products

Methods

Results

Conclusion