Evaluation of tumor necrosis factor alpha and interlukine-11 in a sample of aborted women infected with human Cytomegalovirus

Abstract

This study aimed to evaluate the immunological role of TNF-alpha and interlukine-11 for a sample of women infected with HCMV. 65 blood samples were collected from aborted women and 15 blood samples were collected from healthy non-aborted women (as a control group) during the period from 6 April to 12 June from reviews Al-Zahra teaching hospital and Al-Manathira general hospital in Najaf governorate. HCMV IgM, IgG antibody tests were performed by using Enzyme-Linked Immunosorbent Assay (ELISA- Human, Germany).The results showed that the prevalence of IgG and IgM immunoglobulins against HCMV in pregnant women with miscarriages according to the statistical analysis that there is a high significantly difference with a level ≤ P 0.05 in IgG and IgM concentration within different age groups of aborted women compared to the control treatment group of women.The samples under study were also tested to measure the level of TNF-α and IL-11 cytokines by using a quantitative technique the enzyme-linked immunosorbent assay as well, the results showed that there is a hight significant in the concentration of TNF-α protein in aborted women, which reached 8.24 ± 1.58 (pg/ml)., and this was significant at P ≤ 0.003 compared with the control group 5.51 ± 2.96(pg/ml).