Abstract
The present study was conducted to biocontrol in vitro and in vivo of Fusariumoxysporum that cause Fusarium wilt diseases for eggplant plants by using biologicalcontrol agent fungus Trichoderma harzianum. Fourteen isolates from F. oxysporumwere isolated and identified from two fields in Iraq. Pathogenicity test indicated thatall F. oxysporum isolates were pathogenic for eggplant but differed in its level ofpathogenicity. Four of the fourteen isolates from F. oxysporum were selecteddepending on their highest pathogenicity for eggplant plants, F. oxysporum fourisolates F5, F6, F13 and F14 achieved at pre emergence 83.3%, 83.3%, 86.7% and83.3% and at post emergence 90.0%, 90.0%, 83.3% and 76.7% respectively. Invitro, the antagonistic activity evaluation of T. harzianum against F. oxysporum fourisolates revealed that T. harzianum was highly significantly inhibited F. oxysporumfour isolates growth (F5, F6, F13, F14) which recorded the inhibition percentage87.00, 89.00, 89.00, and 96.33% respectively. Greenhouse (In vivo) results of thebiocontrol efficiency of T. harzianum against F. oxysporum showed significantreduction in eggplant Fusarium wilt incidence compared with pathogens controltreatments. The pathogen F. oxysporum treatments with T. harzianum (TF) showedthat all the treatments (TF) achieved high germination rate for eggplant seedscompared with control treatment, while the treatment TF13 was significantlysuperior than all other treatments in number of leaflets, stem height, shoot freshweight, shoot dry weight, root fresh weight, root dry weight and root size whichrecorded 8.67, 19.00cm, 20.33gm, 4.73gm, 12.00gm, 1.53gm, 17.67cm³respectively.
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