Abstract
Objective: Photoacoustic tomography (PAT) and multispectral optoacoustic tomography (MSOT) are evolving technologies that are capable of delivering real-time, high-resolution images of tissues. The purpose of this study was to evaluate the feasibility of using PAT and MSOT for detecting histology in a rabbit tracheal stenosis model. Method: A total of 12 rabbits (9 stenosis and three control) were randomly divided into four groups (A, B, C and D). Each group consisted of three rabbits, which were staged at the first, fourth, and eighth weeks of stenosis progression, respectively. PAT/MSOT images and corresponding histology from these experimental animals were compared, for analyzing the morphologic features and quantitative tracheal measurements in different tracheal stenosis stage. Result: Both the PAT images and corresponding histology indicated the most severe degree of stenosis in group C. MSOT images indicated notable differences in tracheal contents of group B and D. Conclusion: This study suggests that PAT/MSOT are potentially valuable non-invasive modality which are capable of evaluating tracheal structure and function in vivo.
Highlights
Photoacoustic tomography (PAT), is an emerging biomedical imaging modality that combines optical and ultrasonic imaging for the combined benefits of enhanced optical resolution and acoustic penetration depth (Attia et al, 2019; Manohar and Gambhir, 2020)
This rabbit had developed white viscous pus scattered between the soft tissue layer of the anterior neck region and the tracheal incision, causing edematous tissue and displacement of the trachea
computed tomography (CT) (Cao et al, 2020) and magnetic resonance imaging MRI (Elders et al, 2021) are routine techniques for detection of tracheal stenosis, those methods confer a risk of ionizing radiation and/or are limited in their usage at times, as stents or metallic foreign bodies are common contraindications
Summary
Photoacoustic tomography (PAT), is an emerging biomedical imaging modality that combines optical and ultrasonic imaging for the combined benefits of enhanced optical resolution and acoustic penetration depth (Attia et al, 2019; Manohar and Gambhir, 2020). Multispectral optoacoustic tomography (MSOT) is based on the principle of PAT, which is formation of sound waves following light absorption by multiwavelength pulsed laser. Tremendous developments in PAT have been achieved, allowing a wide range of applications in preclinical and clinical research, including imaging of thyroid (Dima and Ntziachristos, 2016; Roll et al, 2019), breast (Xu et al, 2019; Yang et al, 2020), tumors (Li. Multispectral Optoacoustic Tomography et al, 2015; Yamada et al, 2020; Karmacharya et al, 2021; Knorring and Mogensen, 2021; Wang et al, 2021) and skin lesions (Petri et al, 2016; Dahlstrand et al, 2020), as well as functional neuroimaging (Wang et al, 2003; Wu et al, 2019a; Lv et al, 2020). PAT and MSOT could be an accurate imaging modality for assessment of blood vessels diseases in vivo (Yang et al, 2019; Bok et al, 2021; Karlas et al, 2021), highlighting the potential of them as a new tool to evaluate structural and functional information in tubular structures like artery, vein, trachea and bowel
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