Evaluation of total serum lipid values determined by the sulfophospho-vanillin reaction method and the lipid-summation method (author's transl)

Abstract

In the physical screening for the clinical diagnosis of hyperlipemia, total cholesterol values are used rather than total serum lipid values.A simplified method for total serum lipid determination using the sulfo-phospho-vanillin reaction has recently been employed in clinical laboratories. The sum of total cholesterol, triglyceride, and phospholipid was proposed by Cheek, summation method 1, and this method is used to determine the total serum lipid values for the clinical test.We evaluated the total serum lipid values in hyperlipemia by the sulfo-phospho-vanillin reaction method, summation method 1, and another method, summation method 2, in which each value for total cholesterol, triglyceride, and phospholipid are summed.Results:1) The sulfo-phospho-vanillin reaction method shows the specific reaction to higher unsaturated fatty acids but not to saturated fatty acids. Total serum lipid values measured by gravimetry and oxidometry show good correlations when this method is used.2) The mean value of total serum lipid determined by summation method 1 is in good agreement with the values from gravimetry and oxidometry.3) The values obtained by summation method 2 are about 15% lower than those obtained by the standard method, gravimetry.4) In the diagnosis of hyperlipemia the sensitivity is 72.3% in the sulfo-phospho-vanillin reaction method, 71.1% in summation method 1, and 78.0% in summation method 2.We concluded that the values determined by the sulfo-phospho-vanillin reaction method, and the lipid-summation methods proposed by Cheek and developed by us are useful for the physical screening of hyperlipemia.