Abstract
Aim:The aim of this study was to generate composite bone graft and investigate the rabbit fetal osteoblasts adhesion, proliferation and penetration on acellular matrices of cancellous bone.Materials and Methods:Acellular cancellous bone was prepared and developed as in the previous study with little modification. These matrices were decellularized by rapid freeze and thaw cycle. To remove the cell debris, they were then treated with hydrogen peroxide (3%) and ethanol to remove antigenic cellular and nuclear materials from the scaffold. Primary osteoblast cells were harvested from 20 to 22 days old rabbit fetal long and calvarial bone. These cells were cultured and characterized using a specific marker. The third passaged fetal osteoblast cells were then seeded on the scaffold and incubated for 14 days. The growth pattern of the cells was observed. Scanning electron microscope and hematoxylin and eosin staining were used to investigate cells proliferation.Results:The cells were found to be growing well on the surface of the scaffold and were also present in good numbers with the matrix filopodial extensions upto inside of the core of the tissue.Conclusion:Thus, a viable composite scaffold of bone could be developed which has a great potential in the field of bone tissue engineering.
Highlights
Many attempts have been made to develop a suitable bone graft substitute for repair of segmental bone defect
Conclusion: a viable composite scaffold of bone could be developed which has a great potential in the field of bone tissue engineering
The chemicals and cell culture media used for fetal osteoblast cultures were procured from the reputed agencies
Summary
Many attempts have been made to develop a suitable bone graft substitute for repair of segmental bone defect. The disadvantages of autogenous bone grafts are limited availability, harvesting morbidity, and insufficient biomechanical properties [2]. The allogeneic bone graft may be associated with risk of pathogen transmission from donor to host, immunogenicity, and limited availability [3]. Xenogeneic bone grafts consist of skeletal tissue that is harvested from one species and transferred to the recipient site of another species [5]. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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