Evaluation of tissue in repair with natural latex and / or hyaluronic acid in surgical bone defects.

Ana Paula Macedo,João Paulo Mardegan Issa,Yamba Carla Lara Pereira,Otavio Marino Dos Santos Neto,Miliane Gonçalves Gonzaga,Sara Feldman

doi:10.1590/0103-6440202104302

Ana Paula Macedo, João Paulo Mardegan Issa + Show 4 more

Open Access

https://doi.org/10.1590/0103-6440202104302

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Abstract

This study evaluated the bone repair in surgical defects of rats treated with hyaluronic acid (HA) associated or not with Hevea brasiliensis fraction protein (F-1). Bone defect were created in 15 albino Wistar rats divided into 3 groups (n=5): Control group (1) - blood clot; HA group (2) - 0.5% hyaluronic acid; HAF1 group (3) - 0.1% F-1 protein fraction dissolved in 0.5% hyaluronic acid. After 4 weeks, the animals were euthanized and the bone repair was evaluated through histomorphometric analysis, zymography and immunohistochemistry. The neoformed bone area did not show a significant difference (p = 0.757), but there was a tendency for bone trabeculation to increase in the groups HA and HAF1. For immunohistochemically analysis, there was a difference in vascular endothelial growth factor (VEGF) labeling (p = 0.023), being higher in the groups HA and HAF1 than the control group. No significant difference in bone sialoprotein (BSP) (p = 0.681), osteocalcin (p = 0.954), however, significant difference in platelet endothelial cell adhesion molecule-1 (CD-31) (p = 0.040), with HAF1 group being significantly lower than the control. For zymographic analysis, there was no significant difference for metalloproteinase-2 (MMP-2) (p = 0.068), but there was a tendency to increase MMP-2 in the HA group. Despite the influence on angiogenic factors and the apparent tendency for greater trabeculation in the HA and HAF1 groups, there was no significant difference in the area of newly formed bone tissue in the analyzed period.

Highlights

The bone repair has aroused great interest in experimental research, since bone defects, congenital or caused by diseases, trauma or surgeries, do not heal spontaneously, being a challenge for orthopedic and dental practice[1]
These animals were randomly divided into 3 groups with a waiting period until the sacrifice of 4 weeks according to the filling of the bone defect: control blood clot; HA - 0.5% hyaluronic acid in buffered saline; HAF1-0.1% F-1 dissolved in 0.5% hyaluronic acid in buffered saline
The rubber particles are mainly composed of cis-1,4-polyisoprene enrobed in a monolayer of protective phospholipid–protein membrane

Summary

Introduction

The bone repair has aroused great interest in experimental research, since bone defects, congenital or caused by diseases, trauma or surgeries, do not heal spontaneously, being a challenge for orthopedic and dental practice[1]. Several strategies have been developed to promote bone regeneration, such as the use of bone grafts, implants of various types, guided tissue regeneration barrier, growth factors, and especially bone morphogenetic proteins – BMPs [2,3,4] Many of these materials have shown only limited efficacy for bone repair, and some have specific disadvantages[1,5,6,7]. The natural latex integrated causing better healing and reported bone growth without any evidence of hypersensitivity or inflammation. This material is promising because it has elasticity, flexibility, low cost, biocompatibility and ability to promote and accelerate angiogenesis[6,17]. New technologies have been developed to improve the performance of natural latex, for example, the creation of biomembranes with better cell adhesion[6,18]

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