Abstract
Three commercially available latex agglutination test (LAT) kits for the rapid identification of Campylobacter isolates were evaluated against 87 isolates of Campylobacter spp. and 46 strains of non‐Campylobacter bacteria (26 species). The performance of three LAT kits, MicroScreen® Campylobacter (Mercia Diagnostics, Shalford, UK), BBL CampyslideTM (Becton Dickinson Microbiology Systems, Cockeysville, MD, USA) and MERITECTM‐Campy (jcl) (Meridian Diagnostics, Cincinnati, OH, USA), were compared. All sensitivities for the three LAT kits were 100%. Specificities of both MicroScreen® Campylobacter and BBL CampyslideTM were 100%, but specificity of MERITECTM‐Campy (jcl) was only 89. 1/. The detection limit ranges (log CFU/ml) of MicroScreen®. Campylobacter, BBL CampyslideTM and MERITECTM‐Campy (jcl) against five Campylobacter strains were 7.7–9.1, 7.4–8.0 and 8.5–9.4, respectively. BBL CampyslideTM was more sensitive (10X) than the other two LAT kits (p < 0.01). A one‐day procedure was proposed to identify the suspicious colonies on selective agar by performing the LAT kit, catalase, oxidase and morphological observation.
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