Abstract
ABSTRACT The Thin Agar Layer (TAL) method of Kang and Fung 1998 was used to enumerate heat‐injured (55 °C for 10 min) foodborne pathogens. This method involves overlaying 14 mL of nonselective medium (Tryptic Soy Agar, TSA) onto a prepoured, pathogen‐specific, selective medium. The recovery rate with the TAL method was compared with the rate for TSA and pathogen‐specific, selective media. No significant differences occurred between TSA and TAL (P > 0.05) for enumeration of heat‐injured Escherichia coli O157:H7, Listeria monocytogenes, Salmonella typhimurium, Staphylococcus aureus, and Yersinia enterocolitica, and both recovered significantly higher numbers than selective media (P < 0.05). The TAL method is a 1‐step, convenient procedure for recovery of heat‐injured cells.
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