Abstract
Tumor-infiltrating lymphocytes (TILs) were isolated from surgically excised squamous cell carcinoma of the head and neck. Immunohistology showed that the tumor was infiltrated by T-lymphocytes, many of which were activated as judged by the expression of the following surface antigens: HLA-DR, transferrin receptor, and receptors for interleukin 2 (IL2). Fresh TILs were unable to lyse natural killer cell (NK)-resistant and NK-sensitive tumor cell targets in chromium-release cytotoxicity assays. Tumor-infiltrating lymphocytes as well as autologous peripheral blood lymphocytes were cultured in media containing natural IL-2. Tumor-infiltrating lymphocytes proliferated better than autologous peripheral blood lymphocytes and exhibited sustained growth and antitumor effector function in long-term cultures. The IL2-expanded culture of TILs contained mostly CD8+ (suppressor/cytotoxic) T-lymphocytes with the morphology characteristic of lymphokine-activated killer cells. The ability to expand TILs with augmented antitumor activity in long-term cultures indicates that these cells could be therapeutically useful. Potential application of human TILs to adoptive immunotherapy of squamous cell carcinoma of the head and neck is discussed, and recent advances in immunotherapy with IL2-activated TILs are reviewed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
