Abstract
Aim: The study aimed at evaluating in vivo wound healing effect of herbal ointment formulated with 15% w/w Trichodesma zeylanicumm methanolic root extract, plant commonly used as traditional medicine. Methodology: The wound healing potential of T. zeylanicumm was assessed using excision wound model and various biochemical parameters; L-hydroxyl, Hexose amine, Malondialdehyde and Ascorbic acid. Treatments were administered daily topically to three groups of albino rats: Ointment base only, 15% w/w T. zeylanicumm methanolic ointment and Neosporin ointment for 20 days. Results: The results showed wound closure was slow in albino rats treated with 15% w/w T. zeylanicumm methanolic extract ointment but increased gradually to 76.95% on day 17, while there was steady increase for Neosporin ointment treated group (from 36.01% on day 5 to 92.89% on day 17). Wound contraction ability of the herbal ointment was significantly greater p<0.01 on 13 day. The Neosporin ointment treated group had short epithelialization time (19.33±1.53) compared to herbal ointment treated group (21.33±3.06). The levels of mucopolysacharide content in the herbal ointment treated group were significantly decreasing P<0.05(from 2.03±0.11 to 1.17±0.13 on 4 to 12 day respectively), while collagen content levels were significantly increasing P˂0.05 in the herbal ointment treated group (2.59±0.10 and 2.63±0.1 on 8 and 12 day). However, the levels of mucopolysacharide and collagen contents were significantly higher in the Original Research Article Annual Research & Review in Biology, 4(6): 828-839, 2014 829 Neosporin ointment than in the herbal ointment treated group. For the blood plasma samples, the malondialdehyde levels were significantly much higher in the herbal ointment treated group (1.74±0.13) compared to Neosporin ointment treated group (1.55±0.08). The ascorbic content levels were significantly higher in the Neosporin ointment sample (0.92±0.26) compared to herbal ointment treated group (0.64±0.14). Conclusion: The results showed that T. zeylanicumm methanolic root extract has wound healing potential; however, further clinical and toxicological experimentation is needed to scientifically validate its use as a topical ointment.
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