Abstract
ABSTRACTBackgroundRapid detection of a wide range of etiologic agents is essential for appropriate treatment and control of gastrointestinal (GI) infections. A variety of microbial species including bacteria, viruses, parasites, and fungi have been recognized as diarrheagenic enteric pathogens. However, multiplex testing of various targets in a single reaction needs further improvement because of its limitation in species and throughput.ResultsThis study aims at developing and evaluating a DNA microarray-based qualitative multiplexed polymerase chain reaction (PCR) assay, Vibrant GI pathogen panel (GPP), for simultaneous detection of 27 enteric GI pathogenic targets (16 bacteria, 5 viruses, 4 parasites, and 2 fungi) directly from stool specimens. Limits of detection ranged from 102 to 104 cells/mL for bacteria, 102 to 103 cells/mL for parasites, 102 to 103 RNA copies/mL for viruses, and 102 to 103 cells/mL for fungi. Performance characteristics were determined using 27 Quantitative Genomic DNAs, 212 spiked stool specimens, 1067 clinical and archived stool specimens. Overall sensitivity was 95.9% (95% CI 92.4–98.1) and specificity was 100% (95% CI 99.9–100). Polymicrobial detections contained either two or three organisms was 20.2% (35/173) of positive clinical specimens and 3.3% (35/1055) of all clinical specimens.ConclusionThe Vibrant GPP is a comprehensive, high-throughput, and rapid DNA microarray to provide etiologic diagnosis of GI infections in the laboratory setting.
Highlights
Infectious diarrhea is a leading cause of global morbidity and mortality, which contributes to the death of around one million children globally each year [1, 2]
As we presented in this study, have the potential to overcome the above issues and provide new opportunities to detect enteric pathogens
We developed and evaluated the Vibrant GI pathogen panel (GPP), which is a DNA microarray-based qualitative multiplexed polymerase chain reaction (PCR) assay intended for use in simultaneous detection and identification of nucleic acids from multiple GI pathogens directly from the stool samples obtained from individuals with GI infection symptoms
Summary
Infectious diarrhea is a leading cause of global morbidity and mortality, which contributes to the death of around one million children globally each year [1, 2]. A variety of bacteria, viruses, and parasites can cause gastrointestinal (GI) infections that manifest as inflammation of the stomach and intestines [3, 4]. A healthcare practitioner may suspect the infectious agents based upon a person’s recent food and drink, medical history, and/ or recent travel but will not be able to positively identify the pathogen without laboratory testing [5]. Different diagnostic modalities are available to provide qualitative and/or quantitative results but all have inherent limitations. Antigen-based tests provide advanced diagnostic results for diarrheal; not all relevant pathogens have been determined with this method [8]. As we presented in this study, have the potential to overcome the above issues and provide new opportunities to detect enteric pathogens. A variety of microbial species including bacteria, viruses, parasites, and fungi have been recognized as diarrheagenic enteric pathogens. Multiplex testing of various targets in a single reaction needs further improvement because of its limitation in species and throughput
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
