Evaluation of the value of an automatic bacteria identification system and blaOXA-51-like gene amplification in the identification of acinetobacter species

Abstract

Objective To evaluate the value of automatic bacteria identification systems including Phoenix and Vitek2 Compact, as well as blaOXA-51-like gene amplification in the identification of Acinetobacter species compared with amplified ribosomal DNA restriction analysis(ARDRA). Methods A total of 50 non-repeated clinical isolates of Acinetobacter species were collected in PUMCH from 2006 to 2007 and identified by ARDRA, blaOXA-51-like gene amplification, Phoenix and Vitek2 Compact,respectively. Results Compared with ARDRA, the sensitivity and specificity of blaOXA-51-like gene amplification were both 100%. The accuracy rates of Phoenix and Vitek2 Compact were 44% and 56%, respectively. Conclusions The accuracy of phenotypic identification of Acinetobacter species is not ideal, however, blOXA-51-like gene amplification could be used as a fast and reliable method for identification of A. baumannii. ARDRA is recommended in the study of drug resistant mechanisms and homological analysis of Acinetobacter species. Key words: Acinetobacter; Bacterial proteins; Beta-lactamases; Palymorphism, restriction fragment length; Polymerase chain reaction