Evaluation of the use of microcrystalline chitosan and collagen membranes as carriers for the platelet derived growth factor (PDGF-BB) in the presence of amoxicillin

Abstract

The aim of the present study was to evaluate the in vitro release of platelet-derived growth factor (PDGF-BB) from homogeneous microcrystalline chitosan (MCCh), collagen (Coll) and composite microcrystalline chitosan - collagen (MCCh-Coll) membranes in the presence or absence of amoxicillin (Am) and to select the most useful membrane for its practical application. The films were characterized by means of FTIR spectroscopy, swelling studies and SEM images. The kinetics of PDGF-BB release was evaluated by means of the ELISA immunoassay test. Amoxicillin concentration was determined spectrophotometrically at 273 nm. The process of the PDGF-BB growth factor and amoxicillin release from studied membranes was of two-phase nature. The first phase (during the 8 h) was characterized by rapid release whereas in the second phase (up to 10 days) the release was much slower. MCCh-Coll (M4) composite membrane appeared to be the most useful membrane because of the fast release of PDGF-BB as a significant angiogenic growth factor for tissue regeneration and the slow as well as gradual release of the antibiotic (amoxicillin), which carries protective role during regeneration process.