Abstract
Severinia buxifolia (Rutaceae) is a promising source of bioactive compounds since it has been traditionally used for the treatment of various diseases. The present study aimed at evaluating the impact of different solvents on extraction yields, phytochemical constituents and antioxidants, and in vitro anti-inflammatory activities of S. buxifolia. The results showed that the used solvents took an important role in the yield of extraction, the content of chemical components, and the tested biological activities. Methanol was identified as the most effective solvent for the extraction, resulting in the highest extraction yield (33.2%) as well as the highest content of phenolic (13.36 mg GAE/g DW), flavonoid (1.92 mg QE/g DW), alkaloid (1.40 mg AE/g DW), and terpenoids (1.25%, w/w). The extract obtained from methanol exhibited high capacity of antioxidant (IC50 value of 16.99 μg/mL) and in vitro anti-inflammatory activity (i.e., albumin denaturation: IC50 = 28.86 μg/mL; antiproteinase activity: IC50 = 414.29 μg/mL; and membrane stabilization: IC50 = 319 μg/mL). The antioxidant activity of the S. buxifolia extract was found to be 3-fold higher than ascorbic acid, and the anti-inflammatory activity of S. buxifolia extract was comparable to aspirin. Therefore, methanol is recommended as the optimal solvent to obtain high content of phytochemical constituents as well as high antioxidants and in vitro anti-inflammatory constituents from the branches of S. buxifolia for utilization in pharmacognosy.
Highlights
Oxidative stress and autoxidation of human lipids and lipoproteins result in the formation of potentially toxic compounds, causing various human health problems such as aging, cardiovascular disease, diabetes, and cancer [1, 2]
Identification of Plant Material. e branches of S. buxifolia were collected from Phu Loc district. e plants were taxonomically identified by the Botany Research and Development Group of Vietnam (Vietnam). e branch materials were obtained by removing the leaves and thorns, washing under running tap water, and drying at 60°C for 72 h. e resulting dried sample was ground using a mill (Jehmlich, Germany) and used for further experiments
After cooling to room temperature, the absorbance of the sample was measured at 660 nm using a V-730 UV-Vis spectrophotometer (Jasco, USA). e percentage inhibition of protein denaturation was calculated using the following equation, and the results were reported as IC50 values: percentage inhibition(%) Acontrol − Asample × 100, (5) Acontrol where Acontrol is the absorbance of negative control (DMSO) and Asample is the absorbance of the extract
Summary
Oxidative stress and autoxidation of human lipids and lipoproteins result in the formation of potentially toxic compounds, causing various human health problems such as aging, cardiovascular disease, diabetes, and cancer [1, 2]. Ese antioxidant agents have been proven to be a promising medicinal product to prevent oxidative stress, disease, and maintain health and delay aging processes These natural antioxidants are recognized as potential anti-in ammatory agents, which safely protect the human body against in ammation, preventing diseases and disorders caused by in ammation [6,7,8]. These natural antioxidants are recognized as potential anti-in ammatory agents, which safely protect the human body against in ammation, preventing diseases and disorders caused by in ammation [6,7,8]. erefore, nding new and safe natural agents with antioxidant and antiin ammatory activity is the objective of the continued study
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