Evaluation of the toxic potential of cefotaxime in the third instar larvae of transgenic Drosophila melanogaster

Abstract

The present study was carried out to evaluate the toxic potential of cefotaxime in the third instar larvae of transgenic Drosophila melanogaster (hsp70-lacZ)Bg9. Cefotaxime at final concentration of 10, 20, 40, 60 and 80μg/ml was mixed in the diet and the larvae were exposed to the selected doses for 6, 12, 24, 48h. The hsp70 expression, trypan blue exclusion test, in situ histochemical β-galactosidase activity, lipid peroxidation, total protein content, glutathione (GSH) content, glutathione-S-transferase (GST) activity, protein carbonyl content, caspase 3 and 9 activity, apoptotic index and comet assay were taken as parameters for the study. The larvae exposed to 40, 60 and 80μg/ml for 12, 24 and 48h showed a dose and duration dependent significant increase in the activity of β-galactosidase and lipid peroxidation but decrease in the total GSH content as compared to unexposed larvae. The decrease in protein content was observed in the larvae exposed to 40, 60 and 80μg/ml of cefotaxime for 24 and 48h. The larvae exposed to 40, 60 and 80μg/ml of cefotaxime for 24 and 48h showed a dose and duration dependent increase in the tissue damage, GST, caspase 3 and 9 activity, PC content, apoptosis and the DNA tail length (comet assay). The result suggests that the cefotaxime is toxic at 40, 60 and 80μg/ml of doses for the third instar larvae of transgenic D. melanogaster (hsp70-lacZ)Bg9. Cefotaxime at 10 and 20μg/ml was not toxic for any duration of exposure.