Abstract
Relevance. Cryopreserved placenta extract (CPE) increase the resistance of the mucous membrane of the gastrointestinal tract to the damaging effects of nonsteroidal anti-inflammatory drugs. Preventive administration of CPE can reduce the ulcerogenic effect of meloxicam, ibuprofen, diclofenac sodium (DS) and others. There is evidence of CPE's own anti-inflammatory activity, which can be successfully combined with the pharmacological properties of nonsteroidal anti-inflammatory drugs, while improving their safety profile.
 Objective: to characterize the therapeutic activity of the combined use of CPE and DS according to hematological parameters in the model of experimental rheumatoid arthritis (RA).
 Materials and methods. Studies were performed on 28 nonlinear laboratory rats. The rats were divided into 4 groups: I (n = 7) – intact rats; II (n = 7) – rats with experimental RA; ІІІ (n = 7) – rats with experimental RA, treated with DN; IV (n = 7) – rats with experimental RA, treated with DN and CPE. Adjuvant arthritis was modeled by subplantar administration of complete Freund's adjuvant. Treatment was performed from 14 to 28 days. CPE was administered on days 14, 17, 20, 23 and 26, and DS – daily. Blood tests were performed on day 28 of the experiment.
 Results. The combined use of CPE and DS is accompanied by a more pronounced leveling of inflammatory signs by hematological parameters – erythrocyte clotting rate decreased by 72.2% (p<0.001), and the number of leukocytes decreased by 54.81% (p<0.001) relative to rats with adjuvant arthritis without treatment. There was a leveling of signs of anemia of chronic inflammation – the level of hemoglobin and erythrocytes increased (p<0,001) by 17.6% and 36.8%, respectively, relative to rats with adjuvant arthritis without treatment.
 Conclusions. The combined use of CPE and DS is superior in therapeutic activity to monotherapy with this nonsteroidal anti-inflammatory drug of experimental rheumatoid arthritis.
Highlights
Вплив диклофенаку натрію та його комбінації з кріоконсервованим екстрактом плаценти на рівень гемоглобіну та кількість еритроцитів і тромбоцитів периферичної крові щурів з ад’ювантним артритом на 28 добу експерименту без лікування) (n=7)
ConclusionsThe combined use of CPE and DS is superior in therapeutic activity to monotherapy with this nonsteroidal antiinflammatory drug of experimental rheumatoid arthritis
Summary
Експериментальні дослідження in vivo проведені на 28 нелінійних лабораторних щурах-самцях масою 200-220 г, які утримувались в умовах віварію ІПКіК НАН України, розділених на 4 групи:. Комбіноване застосування ДН і КЕП призвело до повного нівелювання ознак анемії хронічного запалення – рівень гемоглобіну та еритроцитів статистично достовірно (р
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