Evaluation of the substitution of poliomyelitis virus for testing virucidal activities of instrument and surface disinfection

J Steinmann,M Eggers,I Rapp,D Todt,E Steinmann,F.H.H Brill,I Schwebke

doi:10.1016/j.jhin.2021.12.022

Abstract

The Global Polio Eradication initiative has the goal to eradicate poliomyelitis worldwide. This means that poliomyelitisvirus type 1 strain LSc 2ab (PV-1) can no longer be used for the evaluation of virucidal activity of chemical disinfectants. This study evaluated murine parvovirus ATCC VR 1346 (minute virus of mice) as suitable surrogate for PV-1 when testing virucidal activity of biocides in instrument and surface disinfectants. Suspension testing in different laboratories with two commercially available active biocidal substances based on glutaraldehyde (0.01-0.25%) and peracetic acid (0.005-0.1%) with an exposure time of 30min was performed. Both pathogens showed comparable susceptibility and dose-dependent reduction of virus titres following German and European Guidelines.

Highlights

In the future, it will no longer be possible to examine the virucidal activity of chemical solutions and commercially available active biocidal substances in suspension with poliomyelitisvirus type 1 strain LSc 2ab (PV-1) due to global efforts for poliomyelitis eradication
A stock solution with the factor 1.25% was prepared immediately before examination by adding 11.16 mL of Lerasept spezial to 50 mL water of standardized hardness (WSH) in all participating laboratories followed by dilution
A dose-dependent reduction of both viruses was observed in all four laboratories with increasing concentrations of GDA (Figure 1A) as well as peracetic acid (PAA) (Figure 1B)

Summary

Introduction

It will no longer be possible to examine the virucidal activity of chemical solutions and commercially available active biocidal substances in suspension with poliomyelitisvirus type 1 strain LSc 2ab (PV-1) due to global efforts for poliomyelitis eradication. PV-1 is used in experimental assays since vaccination of the staff in testing laboratories is feasible and high titres in cell culture can be obtained. PV is a stable, non-enveloped, hydrophilic virus and is used to evaluate activity of strong chemical disinfectants used in human medicine. J. Steinmann et al / Journal of Hospital Infection 122 (2022) 60e63

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Conclusion