Evaluation of the Salivary Oxidative Stress, and Non-Enzymatic Antioxidants Marker in Patients with Rheumatoid Arthritis

Abstract

Rheumatoid arthritis (RA) is a chronic systemic inflammatory autoimmune disease that preferentially affects diarthrodial joints. Oxidative stress is elevated in RA patients implying reactive oxygen species (ROS) are possible mediators of tissue damage. Assess the level of total antioxidant capacity (TAC), malondialdehyde (MDA), and non-enzymatic antioxidants (vitamin E, and C )in the saliva of in patients with rheumatoid arthritis(RA). For the realization of our goals we included sixty rheumatoid arthritis patients (RA) (21 female and 39 male) who were fulfilling the American College of Rheumatology and European League Against Rheumatism 2010 revised criteria for classification of RA ,picked randomly, at the age from 25-57 years, and 60 as age matched control group, who attended to Tikrit Teaching Hospital / Tikrit / Iraq during the period from January 2017 to March 2018. Stimulated saliva specimens were collected at the morning. TAC of saliva was evaluated by spectrophotometric assay. Statistical comparisons were performed using Student’s t-test, by SPSS 13. Levels of salivary pH, flow rate, TAC, vit E and C were decreased significantly, whereas the salivary level of MDA were increased significantly in RA patients when compared with healthy controls. There was an increased oxidative stress and a low antioxidant status in patients with RA. These changes are probably due to efforts for reducing lipid peroxidation and hence to lower tissue damage.