Abstract
Between October 2020 and June 2021, 200 cows were screened for subclinical mastitis using the California mastitis test and the White Side test, and Ushir (Vetive-Ria zizanioides) (Linn.) was tested for its antimicrobial activity against mastitis pathogens. A positive control, namely the antibiotic ciprofloxacin, was used to compare the in vitro effectiveness of Vetiveria root oil. In the experimental investigation, the lowest bactericidal concentration against E. coli was recorded at 12.5%, whereas the minimum inhibitory concentration of E. coli and S. aureus were both observed at this level. The largest inhibitory zone was measured against S. aureus, whose lowest zone of inhibition was measured against, which had a mean diameter of 10.67 ± 0.81 mm. E. coli had a mean diameter of 10.89 mm. As a result, the antimicrobial efficacy of Vetivera root oil was found to be effective in controlling subclinical mastitis.
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