Evaluation of the reliability of SE33 locus in forensic DNA identification in Turkish Population

Abstract

Forensic DNA studies is a powerful tool in criminal investigations and paternity test. In recent years, STR (Short Tandem Repeat) DNA loci are being used more and more in forensic identification all over the world. In this context, polymorphism rates and frequency of the SE33 region, which is an autosomal STR locus, is aimed to be determined about its reliability. Evaluations were made on the samples of 500 individuals who did not have close relatives. Genomic DNA isolation was carried out by DNA isolation kits (EZ-1 investigator kit and Power Plex Fusion 6C Direct kit) previously by the authorized staff of İzmir Forensic Medicine Group Presidency. SE33 STR locus amplification was achieved with the Powerplex Fusion 6C kit from the isolated DNA. In the study, homozygote genotype rate was 5.4%, heterozygote genotype rate was 94.6%, power of discrimination (PD) 0.992 and matching probability (PM) was 0.008. The lowest frequency alleles were determined as 7,10,16.3,17.2,23,24,29, 34.2,35.2,37 alleles with 0.1%. It is seen that SE33 STR locus is a strong marker in forensic identification compared to other studies in terms of power of discrimination and matching probability.