Abstract
Viral infections increase the risk of developing allergies in childhood, and disruption of mucosal homeostasis is presumed to be involved. However, no study has reported a role for viral infections in such disruption. In this study, we clarified the mechanism of immunoglobulin A (IgA) overproduction in viral infections. Autopsies were performed on 33 pediatric cases, IgA and interferon (IFN)β levels were measured, and histopathological and immunohistochemical examinations were conducted. Furthermore, we cultured human cells and measured IFNβ and IgA levels to examine the effect of viral infections on IgA production. Blood IgA levels in viral infections were higher than in bacterial infections. Moreover, IFNβ levels in most viral cases were below the detection limit. Cell culture revealed increased IgA in gastrointestinal lymph nodes, especially in Peyer’s patches, due to enhanced IFNβ after viral stimulation. Conversely, respiratory regional lymph nodes showed enhanced IgA with no marked change in IFNβ. Overproduction of IgA, identified as an aberration of the immune system and resulting from excessive viral infection-induced IFNβ was observed in the intestinal regional lymph nodes, particularly in Peyer’s patches. Further, increased IgA without elevated IFNβ in the respiratory system suggested the possibility of a different mechanism from the gastrointestinal system.
Highlights
Blood total immunoglobulin A (IgA) levels in the autopsy cases were compared among the viral infection, bacterial infection, and non-infection groups
The present study aimed to investigate whether differences in location between various lymph nodes infected with viruses might affect IgA production
The results suggested that in gastrointestinal lymph nodes, including intestinal Peyer’s patches, IgA production increased via excessive IFNβ expression resulting from viral infection, thereby disrupting the homeostatic environment of lymph nodes to play a role in immunological aberrations such as allergies
Summary
Object intranasally infection of influenza virus nasal wash ELISA. Subcutaneous immunizationof influenza virus oral inoculation and challenge of rotavirus serum [19]. Male and female canine distemper virus vaccination via intranasal route mouse unidentified canine distemper virus vaccination via oral route mouse. A total of 33 pediatric autopsy cases (16 males and 17 females) aged 0–12 years (median, years) was investigated. The following non-infection cases were investigated as controls: 2 cases of drowning; 2 cases of hyperthermia (heat stroke); 2 cases of fire fatality; 1 case of asphyxia; case of acute circulation failure; and 1 case of blunt force injury. The following cases were excluded from the present study: cases of chronic infections (e.g., chronic hepatitis), cases of malignant tumors, cases of blood dyscrasia (e.g., leukemia), and cases prescribed any steroid known to be associated with low IgA levels from the perspective of clinical medicine
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
