Abstract
Purpose of the study. To evaluate the prognostic significance of biological factors VEGF-A, sVEGF-R1, VEGF-D, FGF, EGF, EGFR, IGF-1, IGF-2, IGFBP-1, IGFBP-2, somatotropin-releasing factor (GHRH) in kidney tissues (tumour tissue, tissue of the perifocal zone and conditionally intact tissue) in local and generalized clear cell renal cancer using ROC analysis.Materials and methods. Two groups of patients were included in the study. Group 1 comprised 50 patients with local kidney cancer (T1–3N0M0), while group 2 comprised 50 patients with metastatic kidney cancer (T1–4N0M1). 10% cytosolic fractions of the kidney tumour tissue were examined. The content of growth factors — somatotropinreleasing factor (GHRH), somatotropin-releasing factor (GHRH) — was determined by the ELISA assay using standard test systems. An assessment of prognostically unfavourable factors that significantly affect the generalization of the tumour process was carried out using binary logistic regression and ROC analysis.Results. The performed ROC analysis revealed diagnostically significant progression biomarkers and their critical values for clear cell renal cancer (for conditionally intact tissue, these values were: VEGF-A ≤ 9107.9 pg/g of tissue; VEGF-R1 ≤ 122.8 ng/g of tissue; FGF ≤ 364.7 pg/g of tissue; IGF-2 ≤ 148 ng/g of tissue; for perifocal tissue, VEGF-A ≤ 5839.6 pg/g of tissue; for tumour tissue, VEGF-A > 9622.5 pg/g of tissue, FGF ≤ 435.1 pg/g of tissue, somatotropin-releasing factor (GHRH) ≤ 158.6 ng/g of tissue). The obtained data contribute to optimization of the disease prognosis.Conclusion. It is established that the most prognostically significant markers of clear cell renal cancer progression include VEGF-A, FGF, somatotropin-releasing factor (GHRH), which can serve as an additional criterion for the differential diagnosis of progression and monitoring of clear cell renal cancer.
Highlights
В связи с этим для математической оценки прогностической значимости исследуемых факторов роста при локальном и генерализованном светлоклеточном раке почки было проведено построение и сравнение ROC-кривых для каждого из биомаркеров (VEGF-А, sVEGF-R1, VEGF-D, FGF, EGF, EGFR, IGF‐1, IGF‐2, IGFВР‐1, IGFВР‐2, СТГрилизинга)
Vascular endothelial growth factor overexpression is correlated with von Hippel-Lindau tumor suppressor gene inactivation in patients with sporadic renal cell carcinoma
В. Уровень некоторых факторов роста семейства VEGF при светлоклеточном раке почки
Summary
С помощью ROC-анализа оценить прогностическую значимость биологических факторов VEGF-А, sVEGF-R1, VEGF-D, FGF, EGF, EGFR, IGF‐1, IGF‐2, IGFВР‐1, IGFВР‐2, СТГ-рилизинга в тканях почки (ткань опухоли, ткань перифокальной зоны и условно интактная ткань) при локальном и генерализованном светлоклеточном раке почки. С помощью ROC-анализа обнаружены диагностически значимые биомаркеры прогрессии и их критические значения при светлоклеточном раке почки (для условно интактной ткани эти значения VEGF-A ≤ 9107,9 пг/г ткани, VEGF-R1 ≤ 122,8 нг/г ткани, FGF ≤ 364,7 пг/г ткани, IGF‐2 ≤ 148 нг/г ткани, для ткани перифокальной зоны VEGF-A ≤ 5839,6 пг/г ткани и для ткани опухоли VEGF-A > 9622,5 пг/г ткани, FGF ≤ 435,1 пг/г ткани, СТГ ≤ рилизинг‐158,6 нг/г ткани). Информация о финансировании: работа проведена при поддержке федерального государственного бюджетного учреждения «Национальный медицинский исследовательский центр онкологии» Министерства здравоохранения Российской Федерации. Для цитирования: Шевченко А.Н., Бреус А.А., Нескубина И.В., Дженкова Е.А., Филатова Е.В., Швырёв Д.А.
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